We successfully conducted Hoffman Winston protocol today in the Jiang Lab! It took a while for us to get everything needed for the protocol. I’m glad it worked.
We successfully conducted Hoffman Winston protocol today in the Jiang Lab! It took a while for us to get everything needed for the protocol. I’m glad it worked.
I’m excited that the collaborative project between me and Joe Armstrong from Maitreya Dunham’s lab is out on biorxiv!
This is an expected discovery when using URA3 as a reporter gene to assay mutation rates and spectra using large population of cells.
We found some mutants that are able to survive on 5-FOA without URA3 mutations which led to the discovery of an interesting
category of URA6 mutants that can survive in various conditions where WT and URA3 mutants can’t.
We sent our first PCR sample to plasmidsaurus for sequencing today! Can’t believe they can sequence up to 25kb linear fragments for $15, without cloning. They use the Nanopore long-read sequencing, with 99% accuracy. This is way cheaper than Sanger sequencing and way simpler, with little compromise in accuracy. Can’t wait to get our results back.
Welcome Tianyu — the first official member of the lab!
Pengyao signed the offer to officially join Center for Mechanisms of Evolution at Arizona State University as an assitant professor starting in January 2024!